发明名称 |
Water-soluble phosphinothiol reagents |
摘要 |
Water soluble reagents and methods for the formation of an amide bond between a phosphinothioester and an azide in an aqueous medium. The phosphinothioester is generated using a water-soluble phosphinothiol reagent. This reaction allows formation of an amide bond between a wide variety of chemical species including amino acids, peptides or protein fragments in an aqueous solution. Of particular interest, this reaction allows for the formation of an amide bond in a physiological setting. In a specific embodiment, this invention provides reagents and methods for peptide ligation in an aqueous medium. The reaction eliminates the need for a cysteine residue and is traceless leaving no residual atoms in the ligated peptide product. |
申请公布号 |
US8410247(B2) |
申请公布日期 |
2013.04.02 |
申请号 |
US20090546249 |
申请日期 |
2009.08.24 |
申请人 |
RAINES RONALD T.;TAM ANNIE;SOELLNER MATTHEW B.;WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
RAINES RONALD T.;TAM ANNIE;SOELLNER MATTHEW B. |
分类号 |
C07C231/10;C07F9/50;C07K1/02;C07K1/04 |
主分类号 |
C07C231/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|