Verfahren zur Herstellung von Penicillin spaltenden Enzympraeparaten

摘要

Penicillin-splitting enzymes are produced by culturing penicillin-splitting bacteria e.g. Escherichia coli under aerobic conditions in a nutrient medium substantially free from fermentable carbohydrate to which ammonium phenylacetate is gradually added during the fermentation to a final concentration of 0.12 to 2.0% w/v. based on the total volume of the fermentation medium.ALSO:An enzyme preparation obtained by the aerobic cultivation of Escherichia coli (see Division C3) is used in conventional manner for splitting penicillins to produce 6-amino penicillanic acid.