| 摘要 |
The present invention relates to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to express at least one nucleic acid molecule encoding a polypeptide with dihydroxyacid dehydratase (DHAD) activity, wherein said polypeptide is at least about 70% identical to a polypeptide selected from SEQ ID NOs: 81-82, 96, 98, 100-101, 107-117, 119-120, 122, 128, 130, 142, 149, 154-156, and 163-166. |
