METHODS FOR IDENTIFYING LEUKEMIA STEM CELLS AND DISTINGUISHING THEM FROM NORMAL HEMATOPIETIC STEM CELLS IN PATIENTS WITH ACUTE MYELOID LEUKEMIA: USES IN DIAGNOSIS, TREATMENT, AND RESEARCH

摘要

Using the methods of the present invention, intermediate (int) levels of aldehyde dehydrogenase (ALDH) activity reliably distinguished leukemic CD34+CD38− cells capable of engrafting immunodeficient mice, from residual normal hematopoietic stem cells that exhibited relatively higher ALDH activity. Minimal residual disease (MRD) detected during complete remission was enriched for the CD34+CD38−ALDHint leukemic cells, and the presence of these cells after therapy highly correlated with subsequent clinical relapse. The methods of the present invention can distinguish normal from leukemic CD34+CD38− cells, and identifies those AML cells associated with relapse. Methods of prediction of relapse of AML patients and methods of treatment are also provided.