<p>A method of wound prognosis comprising the steps of: determining whether a weighted average level of human neutrophil elastase (hNE) and one or more matrix metalloproteinase (MMP) in a sample of wound fluid from a wound exceeds a predetermined threshold value for said weighted average; and assigning said wound to a non-healing category if said weighted average exceeds said predetermined threshold value for said weighted average. Also provided are devices for performing the method.</p>