发明名称 |
CELL CULTURE SUBSTRATE COMPRISING A LAMININ AND A CADHERIN |
摘要 |
The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11 ), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11 ) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein. |
申请公布号 |
CA2849244(A1) |
申请公布日期 |
2013.03.28 |
申请号 |
CA20122849244 |
申请日期 |
2012.09.19 |
申请人 |
TRYGGVASON, KARL;RODIN, SERGEY |
发明人 |
TRYGGVASON, KARL;RODIN, SERGEY |
分类号 |
C07K14/78;C07K14/705;C12M3/00;C12N5/00 |
主分类号 |
C07K14/78 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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