METHOD FOR EXTRACTING GREASE FROM FUNGI OR ALGA THROUGH PHYSICAL WALL-BREAKING

摘要

Disclosed is a method for extracting grease from fungi or alga cells rich in grease through physical wall-breaking. Upon ending of fermentation, fungal or algal fermentation broth undergoes separation performed by a separation system, cells are collected, and cell sludge is controlled. The cell sludge undergoes physical wall-breaking through a colloid mill or a high-pressure homogenizer. Secondary ball-milling wall-breaking and demulsification are performed through a ball mill. Finally, feed liquid undergoes separation through a three-phase separator to obtain grease rich in polyunsaturated fatty acids. The present invention uses a physical wall-breaking and extraction method, the process is simple, and the cell wall-breaking efficiency is high. Due to low-temperature and anti-oxidation treatment, active ingredients in the algal and fungal cells can be effectively protected. The product is green and non-toxic, and has no residue. The present invention is applicable to extraction of such grease as the DHA grease, the ARA grease, the EPA grease, the GLA grease, and the ALA grease, and is applicable to industrial production.