Immune detection method for common epitopes of two or more analytes in samples of complex compositions, device, and kit for enabling said immune detection method

摘要

The invention relates to a method for detecting in an assay in a test sample one or more analytes, these analytes having at least one common epitope and one non-common linear or structural epitope. The method comprises the steps of providing a set of primary capture elements immobilized on a support, capable to bind to one or multiple non-common linear or structural epitopes of the one or more analytes in the test sample, and incubating the test sample with the immobilized primary capture elements for allowing binding of the one or more analytes to be detected to the immobilized primary capture elements. The method according to the invention is characterized by the further steps of providing a set of one or more secondary capture elements capable to bind to common epitopes of the one or more analytes to be detected in the test sample and incubating said secondary capture elements with the test sample and the immobilized primary capture elements. Analytes exhibiting common epitopes and having been contained in the test sample and bound to the immobilized primary capture elements after incubation with said secondary capture elements are detected. The invention also encompasses a device comprising a lateral flow device and a kit designed for allowing to perform the inventive method.