Compositions, kits and related methods for the detection and/or monitoring of Pseudomonas aeruginosa

摘要

A region of the 23s rRNA of Pseudomonas aeruginosa has been identified as a preferred target for nucleic acid amplification reactions for detecting that bacterium with high selectivity Pseudomonas aeruginosa transcription-mediated amplification assays, are described which employ (i) a T7 provider oligonucleotide that targets the complement of a sequence in a region of Pseudomonas aeruginosa 23s rRNA corresponding to bases from about 725-825 of the E. coli 23s rRNA reference sequence and (ii) a non-T7 primer oligonucleotide that targets a sequence in the same rRNA corresponding to bases from about 845-950 of the E. coli 23s rRNA.