THERMOSTABLE ASSAY REAGENTS

摘要

There is provided a single-chain fusion protein comprising:(i)a thermostable kinase and(ii)a single-domain antibody or single-domain antibody fragment. There is also provided a method of preparing a single-domain antibody or single-domain antibody fragment, the method comprising:(i) expressing the single- domain antibody or antibody fragment as a single-chain fusion protein with a thermostable kinase, in a host cell such as E. coli; and (ii)purifying the fusion protein from the cytoplasm of the host cell.