METHOD FOR REUSING WATER IN FERMENTED BUTANEDIOIC ACID SEPARATION PROCESS

摘要

The present invention relates to the field of biochemical engineering. Provided is a method for reusing water in a fermented butanedioic acid separation process. Water from the separation process is used in aerobic growth and anaerobic fermentation of E.coli AFP111 to produce butanedioic acid, while a final product is provided with a concentration of 55g/L, a yield of up to 91.6%, and, compared with the result of fermentation using a culture medium prepared with tap water, an 8.5% increase in butanedioic acid concentration. The advantages of the present invention are that evaporated water from the butanedioic acid separation process is recycled and reused, thus water reuse is implemented in industrialized production process of butanedioic acid, and at the same time, that the evaporated water produced during the butanedioic acid separation process comprises small amount of organic acids such as ethanoic acid and methanoic acid, the small amount of organic acids comprised in the evaporated water can serve as a carbon source for gluconeogenesis, thus enzymatic activities of some key enzymes within cells are increased, thereby facilitating anaerobic fermentation of bacterial cells to produce acid.