发明名称 |
Stable production of lentiviral vectors |
摘要 |
The present invention provides new stable packaging cell lines and producer cell lines as well as methods to obtain them, and a new method to produce lentiviral vectors using such cell lines. New methods and packaging cell lines of the invention are generated using a baculo-AAV hybrid system for stable expression of structural and regulatory lentiviral proteins, such system comprising a baculoviral backbone containing an integration cassette flanked by AAV ITR, in combination with a plasmid encoding rep protein. This system allows to obtain a stable integration of the structural and regulatory HIV-1 proteins gag/pol and rev. The system allows to obtain a first intermediate including only the structural and regulatory HIV proteins gag/pol and rev, to be used as starting point to obtain stable packaging cell lines as well as producer cell lines. |
申请公布号 |
AU2011298333(A1) |
申请公布日期 |
2013.03.21 |
申请号 |
AU20110298333 |
申请日期 |
2011.09.01 |
申请人 |
MOLMED SPA |
发明人 |
BOVOLENTA, CHIARA;STORNAIUOLO, ANNA;RIZZARDI, PAOLO;MAVILIO, FULVIO |
分类号 |
C12N15/866;C12N7/02;C12N15/867 |
主分类号 |
C12N15/866 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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