METHOD FOR PRODUCING A BIOLOGICAL TISSUE CONSTRUCT AND USE OF SPECIFICALLY OBTAINED AUTOLOGOUS CELLS

摘要

The method for producing a tissue construct for medical purposes uses endothelial progenitor cells (EPC) that have not been multiply passaged and have a content of EOEC (Early Outgrowth Endothelial Progenitor Cells) and LOEC (Late Outgrowth Endothelial Progenitor Cells). These cells and fibroblasts and/or muscle cells, namely myoblasts, myofibroblasts, smooth muscle cells or their precursors, are seeded as living cells on a matrix or are incorporated into the matrix in order to yield a tissue construct after additional treatment steps. The matrix is preferably a protein preparation, in particular a fibrinogen preparation. Both the cells and the fibrinogen preparation can be obtained from a single blood sample of a patient. Besides its use for the production of bypass materials, prosthetic vessel replacement, tissue patches, conduits and the like, the EOEC-containing EPC culture or suspension is suitable to be used for cell transplantation.