发明名称 |
SYSTEMS AND METHODS FOR BEAD-FREE DETECTION OF NUCLEOTIDES |
摘要 |
Methods and systems of quantifying a target material in solution include detection of a size change of a hybridized nucleic acid complex, without the use of nanobeads. In particular, the examples include providing a plurality of nucleic acid fragments and a species-specific oligonucleotide tags, measuring the size of the nucleic acid fragments and/or oligonucleotides to predetermine a standard distribution of the solution(s), introducing the oligonucleotides in a solution containing nucleic acid target materials and/or non-target materials, and hybridizing the oligonucleotides with the species-specific target material if present in the solution. The size of the nucleic acid complexes in solution are then measured after hybridization, and the presence or non-presence of the species-specific target material is detected and/or quantified by comparing the measured size of the nucleic acid complexes after hybridization to the standard distribution. |
申请公布号 |
WO2013040008(A1) |
申请公布日期 |
2013.03.21 |
申请号 |
WO2012US54818 |
申请日期 |
2012.09.12 |
申请人 |
UNIVERSITY OF NOTRE DAME DU LAC;TANNER, CAROL;RUGGIERO, STEVEN |
发明人 |
TANNER, CAROL;RUGGIERO, STEVEN |
分类号 |
G01N33/48;C12Q1/68;G01N21/59 |
主分类号 |
G01N33/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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