QUANTITATIVE MEASUREMENT METHOD FOR SPECIFIC ANALYTE USING SURFACE PLASMON RESONANCE AND SURFACE PLASMON FIELD-ENHANCED FLUORESCENCE SPECTROSCOPY

摘要

[Problem] To provide a quantitative measurement method for a specific analyte, said method being capable of rapid measurement without the use of a column, thereby eliminating the antigen losses that accompany column processes, and also being capable of reducing the consumption amount of a labeled probe such as lectin. [Solution] After SPR measurement is used to measure a total analyte concentration, a fluorescently labeled probe is used to label a specific analyte, SPFS measurement is used to measure the concentration of said analyte, and the ratio of the amount of the specific analyte to the total analyte amount is computed from the total analyte concentration and the concentration of the specific analyte.