发明名称 |
In vitro hepatic differentiation |
摘要 |
<p>This invention relates to the induction of hepatic differentiation by culturing induced pluripotent stem (iPS) cells in an endoderm induction medium to produce a population of anterior definitive endoderm (ADE) cells and then culturing the population of ADE cells in a hepatic induction medium to produce a population of hepatic progenitor cells, which may be optionally differentiated into hepatocytes. The endoderm induction medium is a chemically defined medium which has fibroblast growth factor activity, stimulates SMAD2 and SMAD3 mediated signalling pathways and SMAD1, SMAD5 and SMAD9 mediated signalling pathways, and inhibits phosphatidylinositol 3-kinase (PI3K) and glycogen synthase kinase 3β(GSK3β); and the hepatic induction medium is a chemically defined medium which stimulates SMAD2 and SMAD3 mediated signalling pathways. These methods may be useful, for example, in producing hepatocytes and hepatic progenitor cells for cell-based therapies or disease modelling.</p> |
申请公布号 |
AU2011294869(A1) |
申请公布日期 |
2013.03.21 |
申请号 |
AU20110294869 |
申请日期 |
2011.08.25 |
申请人 |
CAMBRIDGE ENTERPRISE LIMITED |
发明人 |
VALLIER, LUDOVIC;RASHID, SHEIKH TAMIR;HANNAN, NICHOLAS;CHO, HSIN-HUA |
分类号 |
C12N5/071 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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