METHOD OF PRODUCING INCISTEROL

摘要

FIELD: chemistry.SUBSTANCE: invention relates to organic chemistry and specifically to production of sterol derivatives, and can be used in producing 4-hydroxy-17R-methylincisterol from ergosterol by photoisomeration. When carrying out the method, a weighed portion of ergosterol is dissolved in a weakly polar solvent while illuminating the solution with optical radiation in the near UV range. The obtained solution is chromatographically separated. The fraction which is cytotoxic for HeLa line of cancer cells and human myeloid leukaemia cells is sorbed. The pH is raised to neutral value and the solution is evaporated until a milky appearance is achieved. The weakly polar solvent is added and extracted, and the organic layer is separated on separating funnel. The organic layer is evaporated and dissolved. The obtained solution is separated by analytical HPLC with subsequent purification and separation of incisterol.EFFECT: invention enables to obtain a 4-hydroxy-17R-methylincisterol preparation by chemical synthesis.10 cl