METHODS FOR PREPARING BIODIESEL USING ANIMAL CELL CULTURE WASTE

摘要

PURPOSE: A method of manufacturing biodiesel is provided to manufacture biodiesel using cultured animal cell waste without a separate waste treatment process. CONSTITUTION: A method of manufacturing biodiesel from cultured animal cell salvage comprises a first extracting step of adding a polar organic solvent to the collected wet cultured animal cell salvage and stirring to extract lipid; a crude lipid yielding step of layer separating the supernatant and cell pellets and concentrating the supernatant to manufacture concentrate; a second extracting step of adding acid/alcohol solution to yielded crude lipid, refluxing and extracting with a non-polar organic solvent; and a separating step of collecting the non-polar solvent layer and concentrating under the reduced pressure to separating biodiesel. The animal cell is the CHO (Chinese hamster ovary) cell, the BHK (baby hamster kidney) cell, the HEK (human embryonic kidney) cell, and the NS 0 (myeloma cell line) cell. The animal cell is the CHO cell. The polar organic solvent is methanol, ethanol, isopropyl alcohol, acetone or methanol/chloroform solvent mixture. The polar organic solvent is applied at 1-10 times of volume to the volume of the cultured animal cell salvage. The mixing ratio of the methanol/chloroform mixed solution is 1:4 to 4:1. The acid/alcohol solution in the second extracting step is sulfuric acid/methanol solution, sulfuric acid/ethanol solution, anhydrous hydrochloride/methanol solution, anhydrous hydrochloride/ethanol solution, acetyl chloride/methanol, and acetyl chloride/ethanol solution. The acid/alcohol solution in the second extracting step has 1-5 % (w/v) of concentration. The acid/alcohol solution in the second extracting step is applied to 0.2-20 times of volume (v/w) to the crude lipid.