发明名称 |
PHARMACEUTICAL COMPOSITION CONTAINING HUMAN MESENCHYMAL STEM CELL |
摘要 |
Disclosed are a method for producing a frozen pharmaceutical composition prepared from cultured human mesenchymal stem cells and a pharmaceutical composition containing human mesenchymal stem cells. This method is a method for producing a frozen pharmaceutical composition containing human mesenchymal stem cells, which comprises the following steps in this order: (a) adding trypsin to human mesenchymal stem cells in a culture vessel to detach the cells from the surface of the culture vessel; (b) adding a bicarbonate Ringer's solution containing human serum albumin to the detached cells to terminate the reaction with trypsin, and washing the cells with the bicarbonate Ringer's solution containing human serum albumin; (c) suspending the cells in a bicarbonate Ringer's solution containing human serum albumin and dimethyl sulfoxide; (d) putting the resulting suspension in a container which allows freezing of what is contained therein, and sealing the container; and (e) freezing the suspension put in the container. |
申请公布号 |
EP2210608(A4) |
申请公布日期 |
2013.03.20 |
申请号 |
EP20080844376 |
申请日期 |
2008.10.27 |
申请人 |
JCR PHARMACEUTICALS CO., LTD. |
发明人 |
SHIRONO, HIROYUKI;BYUN, YOUNGJIN;KAMEI, SHOICHIRO;IMAGAWA, KIWAMU |
分类号 |
A61K35/28;A61K35/12;A61K35/48;A61K35/50;A61K47/02;A61K47/20;A61K47/42;A61L27/00;C12N5/0775 |
主分类号 |
A61K35/28 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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