发明名称 |
Gene expression technique |
摘要 |
The present invention provides a method for producing heterologous protein comprising: (a) providing a host cell comprising a 2µm-family plasmid, the plasmid comprising a gene encoding a protein comprising the sequence of a chaperone protein and a gene encoding a heterologous protein; (b) culturing the host cell in a culture medium under conditions that allow the expression of the gene encoding the chaperone protein and the gene encoding a heterologous protein; (c) purifying the thus expressed heterologous protein from the cultured host cell or the culture medium. |
申请公布号 |
EP2048236(B1) |
申请公布日期 |
2013.03.20 |
申请号 |
EP20080169442 |
申请日期 |
2004.12.23 |
申请人 |
NOVOZYMES BIOPHARMA DK A/S |
发明人 |
SLEEP, DARRELL;SHUTTLEWORTH, GILLIAN;FINNIS, CHRISTOPHER JOHN ARTHUR |
分类号 |
C12N15/80;C12N5/10;C12N15/67 |
主分类号 |
C12N15/80 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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