Method for examination of air for the presence of biological substances by means of removable multi-well strips on which an immunological assay is performed directly

摘要

The method comprises conducting air by a collecting device comprising a sample carrier (1) for 100 l/minute and performing prolonged collecting process for less than 1 hour, where the biological substances are collected at the sample carrier, and determining the biological substances by a immunologic detection method. The sample carrier is taken out from the collecting device to perform the collecting device, and the immunologic detection method is performed in the sample carrier. The detection of the biological substances takes place using antibodies. The method comprises conducting air by a collecting device comprising a sample carrier (1) for 100 l/minute and performing prolonged collecting process for less than 1 hour, where the biological substances are collected at the sample carrier, and determining the biological substances by a immunologic detection method. The sample carrier is taken out from the collecting device to perform the collecting device, and the immunologic detection method is performed in the sample carrier. The detection of the biological substances takes place using antibodies. The collecting process occurs in an inner space, where a reference measurement is carried out outside the closed space. During the collecting process, the biological substances are collected from 2000 l air. The collecting process is performed filterless directly in the sample carrier, and the sample carrier is media-impermeable for air. The sample carrier comprises micro reaction vessels (2) that are arranged next to each other to form a stripe. The immunologic detection method comprises a negative control, which gives a statement over the correctness of the collecting and detection method, and/or, for control, the supplementary micro reaction vessel is provided. The detection of the biological substances is made by an enzymatic color reaction. Independent claims are included for: (1) a collecting device for collecting biological substances in air for the investigation of biological substances; and (2) an immunologic method such as enzyme-linked immuno sorbent assay to detect antigens from air.