GENETIC RECOMBINANT ECARIN AND PROCESS FOR PREPARING THE SAME

摘要

The present invention is directed
to a recombinant ecarin protein that specifically
activates prothrombin, said protein being efficiently
prepared by the genetic engineering technique comprising
the steps: (1) culturing a transformant microorganism or
animal cell transformed with an expression vector in which
a gene encoding ecarin is incorporated to the downstream of
a promoter so as to produce and accumulate ecarin in
culture supernatant or within said transformant and
recovering the produced ecarin; and (2) purifying a
solution containing the recovered ecarin to obtain purified
ecarin. The present invention allows for production of
recombinant ecarin on an industrial scale.