摘要 |
The present invention relates to a basal medium for spheroid formation of adipose-derived stem cells, comprising a substrate; and a chitosan film faulted on a surface of the substrate, wherein the chitosan film comprises chitosan with 60-90% degree of deacetylation, and the chitosan film has a surface roughness defined by a height difference, measured between a highest position and a lowest position thereof, of 1-25 nm. In addition, the present invention further provides a method for inducing the spheroid trans-differentiating into neural lineages by using the basal medium of the present invention.
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