| 摘要 |
The present invention relates to a method of rapidly detecting microorganisms using nanoparticles, and more particularly to a method and device of rapidly detecting microorganisms by adding, to the microorganisms to be detected, nanoparticles having immobilized thereon an antibody that binds specifically to the microorganisms to be detected, subjecting the mixture to an immune reaction to form a reaction solution, passing the reaction solution through a microorganism-concentrating film to concentrate the microorganisms, capturing microorganisms, which was immune-reacted with the antibody-immobilized nanoparticles, by a microorganism-capturing filtration membrane, and determining the presence and concentration of the microorganisms. The present invention detects microorganisms using nanoparticles having immobilized thereon an antibody that binds specifically to the microorganisms to be detected, so that the presence and concentration of the microorganisms can be determined in a more effective and simpler manner than a conventional detection method, and the inventive method is effective in detecting a small amount of microorganisms owing to high sensitivity. |
