<p>This particle detection device is a device that detects biological particles. The particle detection device is provided with: a collection unit (20) that collects particles on a collection substrate (71); a fluorescence detection unit (30) that irradiates excitation light toward the particles collected on the collection substrate (71) and receives the fluorescence emitted from the particles; and a cleaning unit (50) that removes the particles from the collection substrate (71) at a refreshing position that is apart from the collection/heating position at which particles are collected on the collection substrate (71) by the collection unit (20) and the detection position at which the fluorescence is received by the fluorescence detection unit (30). The particle detection device performs particle detection with high accuracy due to the above-described configuration.</p>