| 摘要 |
<p>A disposable biosensor for determining the content of hemoglobin and hematocrit in a sample of whole blood that includes a laminated strip with a first and second end, at least a reference, a working electrode and a blank electrode embedded in the laminated strip, a capillary channel with an open path for receiving the blood sample beginning from the first end and connecting to a vent opening spaced from the first end, the open path being sufficiently long to expose the reference electrode, the working electrode and the blank electrode to the blood sample, and conductive contacts located at the second end of the laminated strip. The laminated strip has a base layer with a conductive coating, a reagent holding layer, a channel forming layer and a cover layer having an inlet notch at the first end and a vent opening at the end of the capillary channels . The working electrode contains a reagent sensitive to hemoglobin or hemotocrit. The blank electrode is used to measure oxidizable species in the fluid sample and to correct the current signal of the working electrode. The construction of the biosensor allows accurate measurement of the impedance of the fluid sample, which is used to further correct the current signal of the working electrode. The capillary channel is constructed to have a small volume.</p> |
