发明名称 |
BACTERIAL CELL COMPONENT-UNRESPONSIVE MODEL MOUSE |
摘要 |
A knockout mouse which is unresponsive to peptidoglycan, a lipoprotein/lipopeptide and the like, and is useful for elucidating the contribution of individual members of the TLR family to a signaling stimulated with bacterial cell components in vivo, in particular, the role of TLR2 and MyD88 in vivo. A bacterial cell component-unresponsive knockout mouse is generated by a process comprising the steps of: a targeting vector is constructed by replacing a whole or a part of a gene fragment of an exon region containing a cytoplasmic region of TLR2 or MyD88 gene and the like with a plasmid having a poly A signal and a marker gene; the targeting vector is introduced into an embryonic stem cell; the targeting embryonic stem cell having a homologously recombined TLR2 or MyD88 gene is microinjected into the blastocyst of a mouse and the blastocyst is put back into the uterus of a recipient mouse. |
申请公布号 |
CA2509345(C) |
申请公布日期 |
2013.03.12 |
申请号 |
CA20002509345 |
申请日期 |
2000.01.13 |
申请人 |
INDEPENDENT ADMINISTRATIVE INSTITUTION JAPAN SCIENCE AND TECHNOLOGY AGEN CY |
发明人 |
AKIRA, SHIZUO;TAKEUCHI, OSAMU;TAKEDA, KIYOSHI |
分类号 |
C12Q1/02;C07K14/705;C12N15/85;C12N15/89;C12Q1/00;G01N33/50 |
主分类号 |
C12Q1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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