发明名称 |
Reagents utilizing a serinol scaffold for labeling synthetic oligonucleotides |
摘要 |
Novel CE-phosphoramidites and CPG reagents have been synthesized from a serinol backbone. These reagents are useful to introduce functional groups or directly label oligonucleotides. The versatile serinol scaffold allows for labeling at any position (5′ or 3′ termini, or any internal position) during automated DNA synthesis. Multiple labels or functional groups can be achieved by repetitive coupling cycles. Optimal spacer arms and protected label moieties have been specially designed. Further, the natural 3-carbon atom internucleotide phosphate distance is retained when inserted internally. |
申请公布号 |
US8394948(B2) |
申请公布日期 |
2013.03.12 |
申请号 |
US20100891888 |
申请日期 |
2010.09.28 |
申请人 |
NELSON PAUL S.;MACKIE HUGH;MURPHY ANDREW;GLEN RESEARCH CORPORATION;NELSON BIOTECHNOLOGIES, INC. |
发明人 |
NELSON PAUL S.;MACKIE HUGH;MURPHY ANDREW |
分类号 |
C07H21/00;C07F9/22 |
主分类号 |
C07H21/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|