MASS PRODUCTION METHOD FOR DEVELOPING TRANSGENIC PLANT BY USING SOMATIC EMBRYO(INCUDING SOMATIC EMBRYODENIC CALLUS) IN ROSE SWEET YELLOW

摘要

PURPOSE: A method for producing a large amount of transgenic plants using somatic cell embryo of Sweet Yellow is provided. CONSTITUTION: A method for producing a large amount of transgenic plants using rose 'Sweet Yellow' somatic cell embryo and embryogenic callus comprises: a step of co-culturing somatic cell embryo GUS gene-transformed Agrobacterium tumefaciens and GFP gene-transformed Agrobacterium tumefaciens; a step of culturing the co-cultured somatic cell embryo or embryogenic callues in an embryo selection medium containing 250mg/L^-1 of cefotaxim; a step of culturing the somatic cell embryo or embryogenic callus in a medium containing cefotaxim solely or together with PPT(phosphinothricin); a step of culturing a cultured shoot in a medium; a step of culturing the shoot in a shoot growth medium; a step of culturing 1-1.5cm of shoots in a multi-shoot growth medium; and a step of culturing the multi-shoots in a root inducing medium.