| 摘要 |
<p>Provided is a method for detecting target particles in a biosample containing pancreatic juice, said method enabling the detection in a solution that has a lower concentration or number density of the target particles than by conventional photoanalysis techniques. This method comprises: a probe-binding step for preparing a sample solution, which contains a biosample containing pancreatic juice and a fluorescent probe capable of binding to target particles, and binding the target particles in the biosample to the fluorescent probe; and a calculation step for calculating the number of molecules of the target particles bound to the fluorescent probe by the scanning molecule counting method. The light emission properties of emitted light differ between a state where the fluorescent probe is bound to the target particles and a state where the fluorescent probe is in an unbound state. When bound to the target particles, the fluorescent probe emits fluorescence having a wavelength of 600 nm or greater.</p> |
