发明名称 |
Compositions and Methods For High Fidelity Assembly of Nucleic Acids |
摘要 |
Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid. In certain embodiments, the plurality of blunt-end double-stranded nucleic acid fragments can be provided by: releasing a plurality of oligonucleotides synthesized on a solid support; and synthesizing complementary strands of the plurality of oligonucleotides using a polymerase based reaction. |
申请公布号 |
US2013059296(A1) |
申请公布日期 |
2013.03.07 |
申请号 |
US201213592827 |
申请日期 |
2012.08.23 |
申请人 |
JACOBSON JOSEPH;SCHINDLER DANIEL;LAWTON SCOTT S.;GEN9, INC. |
发明人 |
JACOBSON JOSEPH;SCHINDLER DANIEL;LAWTON SCOTT S. |
分类号 |
C12P19/34;C12Q1/68;C40B40/06;C40B50/02;C40B60/14 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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