NOVEL PCR METHOD FOR REDUCING NON-SPECIFIC AMPLIFICATION USING PHOTOLABILE COMPOUND

摘要

PURPOSE: A novel PCR method for reducing non-specific amplification using a photodegradable compound is provided to control polymerization. CONSTITUTION: A novel PCR method for reducing non-specific amplification using a photodegradable compound comprises: a step of providing a reactant containing an oligonucleotide primer or a primer pair in which the 3'-hydroxy group is protected by a photodegradable protection group; a step of irradiating the reactant by an electromagnetic wave at 300-450 nm. The photodegradable protection group is selected from a group consisting of a 2-nitrobenzyl derivative, an o-nitrobenzyl oxy derivative, a benzoin derivative, and a benzyl sulfonyl derivative.