摘要 |
<p>The invention relates to a method and device for cultivating attachment-dependent cells in the presence of microcarriers in a flexible cultivation container. In particular, the invention concerns a cultivation method using a flexible, scalable, disposable, substantially gas-permeable and non-liquid-permeable container for expansion of adherent cells on microcarriers. According to the invention, cultivation of the cells is accomplished under orbital shaking at 8 rpm or more than 8 rpm.</p> |
申请人 |
LONZA COLOGNE GMBH;SCHENK, JUDITH;VAN DEN BOS, CHRISTIAN;ROSENBAUM, CLAUDIA;BRILL, SILKE;SAFTA, JADE |
发明人 |
SCHENK, JUDITH;VAN DEN BOS, CHRISTIAN;ROSENBAUM, CLAUDIA;BRILL, SILKE;SAFTA, JADE |