发明名称 |
IMPROVED AAV VECTORS PRODUCED IN INSECT CELLS |
摘要 |
The present invention relates to the production of adeno-associated viral vectors in insect cells. The insect cells therefore comprise a first nucleotide sequence encoding the adeno-associated virus (AAV) capsid proteins, whereby the initiation codon for translation of the AAV VP1 capsid protein is a non-ATG, suboptimal initiation codon. The insect cell further comprises a second nucleotide sequence comprising at least one AAV inverted terminal repeat (ITR) nucleotide sequence; a third nucleotide sequence comprising a Rcp52 or a Rcp40 coding sequence operably linked to expression control sequences for expression in an insect cell; and, a fourth nucleotide sequence comprising a Rep78 or a Rep68 coding sequence operably linked to expression control sequences for expression in an insect cell. The invention further relates to adeno-associated viral vectors with an altered ratio of the viral capsid proteins that provides improved infectivity of the viral particles. |
申请公布号 |
EP1945779(B1) |
申请公布日期 |
2013.03.06 |
申请号 |
EP20060812721 |
申请日期 |
2006.10.19 |
申请人 |
UNIQURE IP B.V. |
发明人 |
URABE, MASASHI;OZAWA, KEIYA;HAAST, SASKIA, JACOBA, PETRONELLA;HERMENS, WILHELMUS, T.J.M.C. |
分类号 |
C12N15/864;C12N5/10;C12N15/35;C12N15/866 |
主分类号 |
C12N15/864 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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