发明名称 |
A METHOD AND A KIT FOR DISCRIMINATING BETWEEN OREORCHIS PATENS AND OREORCHIS COREANA |
摘要 |
<p>PURPOSE: A method and a kit for discriminating Oreorchis patens and Oreorchis coreana Finet are provided to easily discriminate the Oreorchis patens and the Oreorchis coreana Finet by using a direct sequencing method, an SNP-genotyping method, and an RFLP(Restriction fragment length polymorphism) method. CONSTITUTION: A method for discriminating Oreorchis patens and Oreorchis coreana Finet comprises: a step of preparing biological samples from the Oreorchis patens and the Oreorchis coreana Finet; a step of isolating genome DNA from the biological samples; a step of amplifying a DNA sequence containing a GCCGGC sequence, which is a recognition site of a Nael restriction enzyme in an ITS sequence of the Oreorchis coreana Finet; a step of amplifying a DNA sequence containing a GCNGGC sequence, which is an alternative sequence of the Oreorchis patens; and a step of treating the amplified product with NaeI.</p> |
申请公布号 |
KR20130022313(A) |
申请公布日期 |
2013.03.06 |
申请号 |
KR20110085659 |
申请日期 |
2011.08.26 |
申请人 |
JEJU TECHNOPARK |
发明人 |
OH, DAE JU;SONG, GWAN PIL;JUNG, YONG HWAN;PARK, SO HYUN;CHOI, SUN A;KO, MYUNG SUK |
分类号 |
C12Q1/68;C12N15/11 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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