Herein is reported a method for purifying a polypeptide comprising the steps of i) applying a solution comprising the polypeptide to an ion exchange chromatography material, and ii) recovering the polypeptide with a solution comprising a denaturant and thereby purifying the polypeptide, whereby the ion exchange chromatography material comprises a matrix of cross-linked poly (styrene-divinylbenzene) to which ionic ligands have been attached, and wherein the solution comprising the polypeptide applied to the ion exchange chromatography material is free of the denaturant and the polypeptide adsorbed to the ion exchange chromatography material is recovered with a solution comprising a denaturant at a constant conductivity.
申请公布号
WO2013026803(A1)
申请公布日期
2013.02.28
申请号
WO2012EP66135
申请日期
2012.08.17
申请人
F. HOFFMANN-LA ROCHE AG;FALKENSTEIN, ROBERTO;MAGRI, MARIA, LAURA;MEHR, MICHAELA;SCHWENDNER, KLAUS;SPENSBERGER, BERNHARD
发明人
FALKENSTEIN, ROBERTO;MAGRI, MARIA, LAURA;MEHR, MICHAELA;SCHWENDNER, KLAUS;SPENSBERGER, BERNHARD