SCAFFOLDED ASSEMBLY FOR IN VITRO EXPANSION OF HAEMATOPOIETIC STEM CELLS OR PRECURSOR CELLS, AND PERFUSION BIOREACTOR AND BIOREACTION SYSTEM USING SAME

摘要

The present invention relates to a scaffold assembly for in vitro expansion of haematopoietic stem cells or precursor cells, and to a perfusion bioreactor and bioreaction system for in vitro expansion of haematopoietic stem cells or precursor cells using the scaffold assembly. The scaffold assembly and the perfusion reactor using same of the present invention use materials having biocompatibility, chemical compatibility and mechanical compatibility, and comprise nanosheets and a scaffold providing a three dimensional environment for cell growth by means of rapid prototyping, and thus not only is there structural similarity to an osteoblastic niche but also the cells immobilized on the scaffold are not washed away and, at the same time, it is possible to percussion tap to a constant magnitude by means of a pump, and the cells immobilized on the scaffold are exposed to a constant stimulus such that an optimal environment similar to an in vivo environment is provided for the in vitro growth of the haematopoietic stem cells or precursor cells.