PREPARATION METHOD OF VIRUS EXPRESSING ALPHA-GALACTOSE EPITOPE AND VACCINE

摘要

A method for producing anα-Gal-expressing virus having enhanced immune response to viruses, without requiring the use of any enzyme; an influenza virus vaccine having a high effect (antigenicity), which is produced using anα-Gal-expressing virus produced by the method; and others. Specifically disclosed are: a method for producing anα-galactose epitope (Galα1-3Galβ1-4GlcNAc-R:α-Gal hereinafter)-expressing virus, which comprises the steps of: (1) introducing anα1,3- galactosyltransferase gene in an expressible condition into a cell line that does not expressα-Gal to obtain a cell line capable of expressingα-Gal; (2) inoculating a virus into the cell line capable of expressingα-Gal to obtain a cell line infected with a virus; and (3) culturing the cell line infected with the virus to obtain a virus expressingα-Gal from the culture medium..