发明名称 |
METHOD OF PRODUCING RECOMBINANT ADAMTS13 IN CELL CULTURE |
摘要 |
Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities. |
申请公布号 |
SG186935(A1) |
申请公布日期 |
2013.02.28 |
申请号 |
SG20130000799 |
申请日期 |
2011.07.08 |
申请人 |
BAXTER INTERNATIONAL INC.;BAXTER HEALTHCARE S.A. |
发明人 |
GRILLBERGER, LEOPOLD;REITER, MANFRED;FLEISCHANDERL, DANIEL;BRAMBERGER, GREGOR |
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