| 摘要 |
FIELD: biotechnology.SUBSTANCE: invention discloses a method of identification of the elements which have the ability to terminate the transcripts. To identify the termination sequences a reporter system is used intended for transient transfection in the culture of cells and containing bicistronic matrix. The matrix has the following structure: located under the common promoter one after another open frames of reading the marker proteins of cistron 1 and cistron 2, after the last cistron the known transcription terminator is incorporated; between the cistrons the stop codons are incorporated and a site for internal initiation of translation (IRES); the element tested on the ability to terminate the transcripts is incorporated between the stop codons of the cistron 1 and the site for internal initiation of translation, the gene of resistance to antibiotic, which is located in the body of the plasmid, for selection of cells containing this plasmid.EFFECT: method can be used for rapid screening of elements for the ability to break off the transcripts and construct the transgenic constructions containing effectively functioning regulatory elements, when creation of the expression vectors in biotechnology, agriculture, medicine.2 dwg, 3 tbl, 4 ex |
