发明名称 |
PRODUCTION OF PROTEINS BY AUTOPROTEOLYTIC CLEAVAGE |
摘要 |
The present invention relates to a process for the production of a desired heterologous polypeptide with a clearly defined homogeneous N-terminus in a bacterial host cell, wherein the desired heterologous polypeptide is autoproteolytically cleaved from an initially expressed fusion protein which comprises a polypeptide with the autoproteolytic activity of an autoprotease Npro of a pestivirus and the heterologous polypeptide by the Npro autoproteolytic activity. |
申请公布号 |
CA2380302(C) |
申请公布日期 |
2013.02.26 |
申请号 |
CA20002380302 |
申请日期 |
2000.08.07 |
申请人 |
BIOCHEMIE GESELLSCHAFT M.B.H. |
发明人 |
RUMENAPF, TILLMANN;THIEL, HEINZ-JURGEN;WINDISCH, JORG;KNAUSEDER, FRANZ |
分类号 |
C12N15/09;C12N15/57;C07K14/54;C07K14/56;C07K19/00;C12N1/21;C12N9/48;C12N9/50;C12N15/62;C12N15/70;C12P21/02;C12P21/06;C12R1/19;C12R1/93 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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