发明名称 |
A MICROENCAPSULATION PROCESS OF SERTOLI CELLS, MICROCAPSULES OBTAINED AND THEIR USE FOR PREVENTION AND CURE OF TYPE 1 DIABETES MELLITUS |
摘要 |
<p>Disclosed is a process for the manufacture of hydrogel-based microcapsules, containing Sertoli cells (SC), comprising the following steps: Production of a homogeneous suspension of SC with purity, in terms of cell composition, higher than 90%, in a saline solution of ultrapure sodium alginate with a concentration ranging from 1 to 5 percent w/x; Aspiration of said suspension at a speed between 10 and 60 m/minute and introduction into a needle-type element; regulation of pressure in the needle-type element; Extrusion of said suspension inside said needle type element through emission of a fluid stream of 3-7 litre/min thereby obtaining a continuous flow of microdroplets showing homogenous dimensions; Introduction of said microdroplets of said flow into an aqueous solution containing divalent cations or polycationic substances with resulting gelification and obtainment of said microcapsules. Futher disclosed is the use of said preparation a sole therapeutic agent in the manufacture of a medicament for prevention and treatment of Type-1 Diabetes Mellitus.</p> |
申请公布号 |
NZ593507(A) |
申请公布日期 |
2013.02.22 |
申请号 |
NZ20090593507 |
申请日期 |
2009.12.18 |
申请人 |
GH CARE INC. D/B/A ALTUCELL |
发明人 |
CALAFIORE, RICCARDO;LUCA, GIOVANNI;CALVITTI, MARIO;BECCHETTI, ENNIO;PUCCETTI, PAOLO;FALLARINO, FRANCESCA;NASTRUZZI, CLAUDIO |
分类号 |
A61K9/50;A61K35/48;A61P3/10 |
主分类号 |
A61K9/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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