BITRANSGENIC BOVINE

摘要

Bitransgenic mammal animal capable of producing humanized milk, expression vector and methods for obtaining it. The bitransgenic non-human animal comprises, inserted in its genome, at least one nucleic acid molecule encoding a heterologous lysozyme and a heterologous lactoferrin operably linked to an expression promoter. Expression of the lysozyme and the lactoferrin is bicystronic, and the lysozyme and the lactoferrin are expressed in the mammary tissue of said non-human mammal. The heterologous lysozyme may be a human lysozyme, for example having an amino acid sequence as set forth in SEQ ID No. 1; and the heterologous lactoferrin may be a human lactoferrin, for example having an amino acid sequence as set forth in SEQ ID No. 2. The milk from the bitransgenic animal comprises human proteins.