The present invention provides a mutagenesis method wherein a nucleic acid molecule is mutagenized with at least one mutagenesis primer in a primer extension reaction and subsequently amplified by rolling circle amplification (RCA). The method involves steps leading to selective amplification of only the mutated strand by a strand-displacing DNA polymerase. Multiple copies of the mutated plasmids are generated during multiple-primed RCA and the resulting DNA is transformed for use. The method is suitable for mutating both single-stranded and double-stranded DNA. The present invention also provides a kit for use in the mutagenesis method.
申请公布号
US2013045507(A1)
申请公布日期
2013.02.21
申请号
US201013517317
申请日期
2010.12.21
申请人
TURUN YLIOPISTO;HUOVINEN TUOMAS;LAMMINMAEKI URPO;BROCKMANN EEVA-CHRISTINE;VEHNIAEINEN MARKUS
发明人
HUOVINEN TUOMAS;LAMMINMAEKI URPO;BROCKMANN EEVA-CHRISTINE;VEHNIAEINEN MARKUS