| 摘要 |
Test method for the detection of at least one analyte from a liquid sample in a sample chamber, comprises fixing at least one receptor molecule on a first surface of a substrate and forming a second surface on the other side, where the support is partially permeable for the liquid sample; forming at least one free volume over a first and second surface, which is limited by the chamber wall; and at least partially forming a liquid column from the free volume. Test method for the detection of at least one analyte from a liquid sample in a sample chamber, comprises fixing at least one receptor molecule on a first surface of a substrate and forming a second surface on the other side, where the support is partially permeable for the liquid sample; forming at least one free volume over a first and second surface, which is limited by the chamber wall, where the sample chamber has at least two openings, and through the first opening the sample liquid flows along a flow gradient over the first and second surface to the second opening; and at least partially forming a liquid column from the free volume, where the flow gradient along the first and second surface takes by pressurization and contains the liquid column. An independent claim is included for a kit comprising the sample chamber containing the support including fixed receptor molecules for performing a binding assay, where the chamber has an inlet and outlet, the top and bottom of the chamber has free space, and the other side of the chamber is connected with the liquid chamber by pressure and other auxiliaries and additives. |
