Modified glyceraldehyde-3-phosphate dehydrogenase from corynebacterium glutamicum and uses thereof

摘要

The present invention relates to enzymatically active glyceraldehyde-3-phosphate dehydrogenase enzymes, which have been modified in their primary structure to alter their coenzyme specificity. In particular, the modified enzymes of the invention exhibit an improved specificity for the coenzyme NADPH which is associated with several advantages in terms of their applicability in biotechnological processes. The invention also provides methods for producing an amino acid, in particular L-lysine or L-threonine, which make use of the modified glyceraldehyde-3-phosphate dehydrogenase enzyme. The invention also relates to the use of the modified glyceraldehyde-3-phosphate dehydrogenase enzyme for biotechnological production processes.