BACTERIA STRAIN Microbacterium testaceum 17B-PRODUCENT OF SITE-SPECIFIC ENDONUCLEASE MteI

摘要

FIELD: biotechnologies.SUBSTANCE: invention represents a bacteria strain Microbacterium testaceum 17B, deposited under the number RNCIM B-10628 in the Russian National Collection of Industrial Microorganisms FGUP GosNIIgenetika, being the producent of the site-specific endonuclease MteI. The invention makes it possible to produce the site-specific endonuclease, which recognises and splits both chains of the nucleotide DNA sequence 5'-G(m5C)G(m5C)NG(m5C)G(m5C)-3'/3'-(m5C)G(m5C)GN(m5C)G(m5C)G-5', where m5C-5-methylcytosine, with production of the single-nucleotide 5'-protruding end, and does not split the methylated DNA sequence 5'-G(m5C)NG(m5C)-3'/3'-(m5C)GN(m5C)G-5'.EFFECT: method improvement.2 dwg, 1 tbl, 3 ex