| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for purifying a polypeptide from a composition comprising the polypeptide and at least one contaminant using the method of ion exchange chromatography. <P>SOLUTION: The method comprises the following steps performed sequentially: (a) binding the polypeptide to an ion exchange material using a loading buffer, wherein the loading buffer is at a first conductivity and pH; (b) washing the ion exchange material with an intermediate buffer at a second conductivity and/or pH so as to elute the contaminant from the ion exchange material; (c) washing the ion exchange material with a wash buffer which is at a third conductivity and/or pH, wherein the change in conductivity and/or pH from the intermediate buffer to the wash buffer is in an opposite direction to the change in conductivity and/or pH from the loading buffer to the intermediate buffer; and (d) washing the ion exchange material with an elution buffer at a fourth conductivity and/or pH so as to elute the polypeptide from the ion exchange material. <P>COPYRIGHT: (C)2013,JPO&INPIT |
