MEDIA FOR THE CULTURE OF WASTE CASTOR-OIL-PLANT CAKE FOR SIMULTANEOUS PRODUCTION OF THE PHYTASE AND THANASE ENZYMES, AND DETOXIFICATION OF THE CASTOR-OIL-PLANT CAKE, USING THE MICROORGANISM PAECILOMYCES VARIOTII AND USING SOLID FERMENTATION, THE ENZY

摘要

The present invention relates to the simultaneous production of thanase and phytase from waste castor-oil-plant cake in solid-state fermentation with Paecilomyces variotii, and also the reduction in the concentration of castor oil after fermentation, thereby promoting the detoxification thereof. The use of enzymes in animal nutrition is known and is the subject of intense interest. The greatest difficulty in terms of wider use of enzymes is still the production cost. The use of waste for animal nutrition represents a viable alternative for this sector, and also for the production of biocatalysts by means of solid fermentation. The present invention determined optimum values for constitution of the culture medium using waste castor-oil-plant cake for production of the phytase and thanase enzymes by means of the solid fermentation of the microorganism Paecilomyces variotii, in such a manner as to produce the greatest possible quantity of enzyme and to achieve the greatest possible performance level thereof. In addition, the present invention enables the detoxification of waste castor-oil-plant cake on account of the degradation of castor oil, the greatest toxic agent thereof, thereby making the waste an ingredient that it is advantageous to use in animal feed.