发明名称 |
PROBE SET FOR IDENTIFICATION OF NUCLEOTIDE MUTATION, AND METHOD FOR IDENTIFICATION OF NUCLEOTIDE MUTATION |
摘要 |
The type of a nucleotide located in a mutation site on a target nucleic acid can be identified by carrying out a fluorescence real-time PCR using a probe set comprising a detection probe and a counter probe, wherein the detection probe comprises an oligonucleotide which comprises a nucleotide sequence containing the mutation site on the target nucleic acid and also containing a nucleotide of interest in the mutation site or a nucleotide sequence complementary to the aforementioned nucleotide sequence, has a fluorescent substance added to the 5'-terminal and a quenching substance added to the 3'-terminal, and has, introduced therein, such a modification that the melting temperature of the probe becomes 3°C or more higher than that of the counter probe, and wherein the counter probe comprises an oligonucleotide which comprises a nucleotide sequence containing a mutation site and also containing a nucleotide that is different from the nucleotide of interest in the mutation site or a nucleotide sequence complementary to the aforementioned nucleotide sequence. |
申请公布号 |
EP2423324(A4) |
申请公布日期 |
2013.02.13 |
申请号 |
EP20100767115 |
申请日期 |
2010.04.22 |
申请人 |
VERTEX PHARMACEUTICALS INCORPORATED |
发明人 |
KISHI, YASUHIRO;KAMIYA, NAOHIRO |
分类号 |
C12Q1/68;A61K31/497;C12N15/09;C12Q1/70 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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